y 320 treatment mice Search Results


320 mics  (ATCC)
99
ATCC 320 mics
320 Mics, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y+320+treatment+mice/Streptococcus+pneumoniae+(Klein)+Chester/10__1128_slash_jcm__42__7__3356___3358__2004-26-9-17
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precision x rad 320  (Precision X-Ray)
98
Precision X-Ray precision x rad 320
Precision X Rad 320, supplied by Precision X-Ray, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse map2  (Becton Dickinson)
90
Becton Dickinson mouse map2
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Mouse Map2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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balb c anncrl 80 balb c mice  (Charles River Laboratories)
86
Charles River Laboratories balb c anncrl 80 balb c mice
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Balb C Anncrl 80 Balb C Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s aureus mrsa atcc 43300  (ATCC)
99
ATCC s aureus mrsa atcc 43300
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
S Aureus Mrsa Atcc 43300, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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animals male wistar rats  (Inotiv)
99
Inotiv animals male wistar rats
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Animals Male Wistar Rats, supplied by Inotiv, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y+320+treatment+mice/C57BL%2F6+Mouse/pmc03158721-25-0-15
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animals male wistar rats - by Bioz Stars, 2026-09
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mic assay  (ATCC)
99
ATCC mic assay
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Mic Assay, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y+320+treatment+mice/MIC/pmc10860147__jm3c01473_si_001-303-213-218
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horseradish peroxidase conjugated goat anti mouse igg  (Bio-Rad)
96
Bio-Rad horseradish peroxidase conjugated goat anti mouse igg
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Horseradish Peroxidase Conjugated Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti gapdh antibody  (Novus Biologicals)
93
Novus Biologicals mouse monoclonal anti gapdh antibody
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Mouse Monoclonal Anti Gapdh Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x-rad 320 x-ray irradiator  (Softex Co Ltd)
90
Softex Co Ltd x-rad 320 x-ray irradiator
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
X Rad 320 X Ray Irradiator, supplied by Softex Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pressure catheter spc-320  (Millar Inc)
90
Millar Inc pressure catheter spc-320
Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and <t>MAP2</t> at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.
Pressure Catheter Spc 320, supplied by Millar Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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buv661 rat anti-mouse cd115  (Becton Dickinson)
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Becton Dickinson buv661 rat anti-mouse cd115

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Image Search Results


Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and MAP2 at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.

Journal: Cerebral Cortex (New York, NY)

Article Title: Cortical Neuron Migration and Dendrite Morphology are Regulated by Carboxypeptidase E

doi: 10.1093/cercor/bhy155

Figure Lengend Snippet: Knockdown of CPE decreases dendritic branching in cortical neurons in vivo and in cultured hippocampal neurons in vitro. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and neurons within cortical layer II/III were analyzed at P7. Representative tracings of dendrites are shown for each condition. Scale bar = 50 μm. (B, C) Quantitation of the number of dendrites (B) and total dendrite length (C) of neurons under each condition. Error bars indicate ± SEM. n (neurons) = 26, CTL shRNA; n = 29, CPE shRNA; n = 50, CPE shRNA+rescue. *P < 0.05, **P < 0.01 as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. (D) Hippocampal neurons were cotransfected with pCAG-mOrange and negative control siRNA (CTL siRNA) or CPE siRNA as indicated at DIV 7. Neurons were fixed and immunostained for GFP and MAP2 at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (E) Sholl analysis of neurons transfected with the indicated siRNA. Gray line indicates P value is at least less than 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (F) Sholl analysis within 60 μm from soma. n (neurons) = 84, CTL siRNA; n = 95, CPE siRNA. *P < 0.05, **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Representative images showing CPE siRNA knockdown efficiency in transfected neurons. Hippocampal neurons were cotransfected with pCAG-mOrange (indicated by arrows) and either CTL siRNA or CPE siRNA at DIV 7, fixed at DIV 10, and immunostained for CPE (indicated by arrowhead in transfected cell). Scale bar = 50 μm. (H) Quantitation of CPE fluorescence intensity in the cell body of transfected neurons. Error bars indicate ± SEM. n (neurons) = 12, CTL siRNA; n = 19, CPE siRNA. ****P < 0.0001 as determined by Student’s t-test.

Article Snippet: Antibodies and Reagents Mouse CPE (BD 610 758) and mouse MAP2 (BD 556 320) primary antibodies were purchased from BD Biosciences (San Jose, CA).

Techniques: In Vivo, Cell Culture, In Vitro, In Utero, Construct, Quantitation Assay, shRNA, Negative Control, Transfection, Fluorescence

Overexpression of the carboxyl terminus of CPE disrupts neuronal migration and dendritic arborization. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and brains were analyzed at E17.5. Representative images of coronal brain sections are shown for each condition. White, transfected cells; gray, Hoechst labeling of nuclei. Scale bar = 50 μm. (B) Quantitation of the percentage of transfected cells in each cortical area. Error bars indicate ± SEM. n = 8, GFP; n = 6, CPE-C10 (carboxyl terminal 10 amino acids of CPE). **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (C) Representative images of transfected cells in the IZ are shown for each condition. Arrows point to unipolar or bipolar cells; arrowheads point to multipolar cells. Scale bar = 20 μm. (D) Quantitation of percentage of unipolar and bipolar cells under each condition. Morphology of transfected cells across the entire IZ was analyzed. Error bars indicate ± SEM. n = 10, GFP; n = 6, CPE-C10. **P < 0.01 as determined by Student’s t-test. (E) Hippocampal neurons were cotransfected with pCAG-mOrange and pEGFP (GFP) or pEGFP-CPE-C10 (CPE-C10) as indicated at DIV 7. Neurons were fixed and immunostained for GFP and MAP2 at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (F) Sholl analysis of neurons expressing GFP or CPE-C10 on dendrite branching. Gray line indicates P value is at least less than 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Sholl analysis within 60 μm from soma. Error bars indicate ± SEM. n (neurons) = 95, GFP; n = 90, CPE-C10. *P < 0.05, **P< 0.01, ***P < 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test.

Journal: Cerebral Cortex (New York, NY)

Article Title: Cortical Neuron Migration and Dendrite Morphology are Regulated by Carboxypeptidase E

doi: 10.1093/cercor/bhy155

Figure Lengend Snippet: Overexpression of the carboxyl terminus of CPE disrupts neuronal migration and dendritic arborization. (A) Mice at E14.5 were electroporated in utero with constructs as indicated, and brains were analyzed at E17.5. Representative images of coronal brain sections are shown for each condition. White, transfected cells; gray, Hoechst labeling of nuclei. Scale bar = 50 μm. (B) Quantitation of the percentage of transfected cells in each cortical area. Error bars indicate ± SEM. n = 8, GFP; n = 6, CPE-C10 (carboxyl terminal 10 amino acids of CPE). **P < 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (C) Representative images of transfected cells in the IZ are shown for each condition. Arrows point to unipolar or bipolar cells; arrowheads point to multipolar cells. Scale bar = 20 μm. (D) Quantitation of percentage of unipolar and bipolar cells under each condition. Morphology of transfected cells across the entire IZ was analyzed. Error bars indicate ± SEM. n = 10, GFP; n = 6, CPE-C10. **P < 0.01 as determined by Student’s t-test. (E) Hippocampal neurons were cotransfected with pCAG-mOrange and pEGFP (GFP) or pEGFP-CPE-C10 (CPE-C10) as indicated at DIV 7. Neurons were fixed and immunostained for GFP and MAP2 at DIV 10. Neurons positive for both GFP and mOrange were assessed for Sholl analysis. Representative mOrange fluorescent images of neurons are shown as inverted black images. Scale bar = 50 μm. (F) Sholl analysis of neurons expressing GFP or CPE-C10 on dendrite branching. Gray line indicates P value is at least less than 0.01 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test. (G) Sholl analysis within 60 μm from soma. Error bars indicate ± SEM. n (neurons) = 95, GFP; n = 90, CPE-C10. *P < 0.05, **P< 0.01, ***P < 0.001 as determined by 2-way ANOVA followed by Sidak’s multiple comparisons test.

Article Snippet: Antibodies and Reagents Mouse CPE (BD 610 758) and mouse MAP2 (BD 556 320) primary antibodies were purchased from BD Biosciences (San Jose, CA).

Techniques: Over Expression, Migration, In Utero, Construct, Transfection, Labeling, Quantitation Assay, Expressing

Journal: iScience

Article Title: Mycobacterium tuberculosis impedes CD40-dependent notch signaling to restrict Th 17 polarization during infection

doi: 10.1016/j.isci.2022.104305

Figure Lengend Snippet:

Article Snippet: To stain lung innate cells, the following surface stain antibodies were used: BUV395 Rat Anti-Mouse CD84 (clone: 1D3/CD84, BD), BUV496 Rat Anti-Mouse I-A/I-E (clone: 2G9, BD), BUV563 Hamster Anti-Mouse CD80 (clone: 16-10A1, BD), BUV661 Rat Anti-Mouse CD115 (clone: T38-320, BD), BUV737 Rat Anti-Mouse DLL4 (clone: 9A1.5, BD), BUV805 Rat Anti-Mouse F4/80 (clone: T45-2342, BD), BV421 Rat Anti-Mouse CD172a (clone: P84, BD), BV421 anti-mouse CD169 (clone: 3D6.112, Biolegend), BV480 Hamster Anti-Mouse CD103 (clone: 2E7, BD), BV570 anti-mouse CD3 (clone: 17A2, Biolegend), BV570 anti-mouse CD19 (clone: 6D5, Biolegend), BV650 anti-mouse/rat XCR1 (clone: ZET, Biolegend), BV711 anti-mouse CD11c (clone: N418, Biolegend), BV750 anti-mouse CD45 (clone: 30-F11, Biolegend), BV786 Mouse Anti-Mouse CD64 a/b (clone: ×54-5/7.1, BD), FITC anti-mouse Ly-6G (clone: 1A8, Biolegend), BB700 Rat Anti-Mouse CD124 (clone: mIL4R-M1, BD), PE anti-mouse Jagged1 (clone: HMJ1-29, Biolegend), PE/Cy5 anti-mouse CD3ε (clone: 145-2C11, Biolegend), PE-Cy7 anti-mouse/human CD11b (clone: M1/70, Biolegend), PE/Dazzle 594 anti-mouse Ly-6C (clone: HK1.4, Biolegend), PE-Cy5 anti-mouse CD24 (clone: M1/69, Biolegend), PE-Cy7 anti-mouse JAML (clone: 4/E10, Novus Biologicals), Alexa 647 Rat Anti-Mouse S100A9 (clone: 2B10, BD), and Alexa 700 anti-mouse/human CD11b (clone: M1/70, Biolegend).

Techniques: Blocking Assay, Purification, Mutagenesis, Recombinant, Lysis, Staining, Sequencing, Electron Microscopy, Negative Control, SYBR Green Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Software